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Alternatively, the inventory culture organisms might be developed in an appropriate liquid medium (i.e., SoybeanCasein Digest Broth or Sabouraud Dextrose Broth) and the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to obtain a microbial count of about 1 × 108 cfu per mL. [NOTEThe estimate of inocul